Does the H tag work as a solubility enhancer? Short fast 1 minute answer!
Regarding the H tag, it did not function as a solubility enhancer tag, but it improved the production levels of target proteins in E. colisimilarly tRead More…
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BioDataTechnology
How can I incorporate solubility enhancers into my fusion tag design? Short fast 1 minute answer!
Fusion tags can be incorporated using different strategies: affinity and solubility tags are set individually or together, and sites for protease cleRead More…
What is the application of the Solubility enhancement tags (SETs)? Short fast 1 minute answer!
The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and sample stability issues and has enabled Read More…
What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred RaidRead More…
How to identify biotinylated proteins? Short fast 1 minute answer!
Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylatRead More…
What is the difference between bioID and biotinylation? Short fast 1 minute answer!
Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuRead More…
How to do biotinylation of fusion protein? Short fast 1 minute answer!
The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification. Including biotin in your culture and induciRead More…
What is Intein Mediated Purification ? Short fast 1 minute answer!
One of the purification methods used in the biotechnology laboratories for better detection and separation is Intein mediated purification techniquesRead More…
Is it possible to crystallize target proteins by fusion of maltose-binding proteins? Short fast 1 minute answer!
Maltose binding protein is one of the revolutions in molecular biology for preparation and separation of the protein from samples. It has been repRead More…
What is the function of maltose/maltodextrin? Short fast 1 minute answer!
Maltose binding protein is one of the revolutions in molecular biology for preparation and separation of the protein from samples. The maltose/malRead More…
What is the function of a maltose binding protein in E coli? Short fast 1 minute answer!
Maltose binding protein is one of the preparation and separation techniques used in biotechnology laboratories. Maltose Binding Protein is a membeRead More…
What is a maltose-binding protein? Short fast 1 minute answer!
Maltose binding protein is one of the preparation and separation techniques used in biotechnology laboratories. Maltose-binding protein ( MBP) is Read More…