Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…

The ability of solubility fusion tags to produce soluble protein is only the first step in the pathway towards protein production. As many of these…

Regarding the H tag, it did not function as a solubility enhancer tag, but it improved the production levels of target proteins in E. colisimilarly…

Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.

One of the purification methods used in the biotechnology laboratories for better detection and separation is Intein mediated purification techniques. The intein-mediated purification system has…

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4

Fusion tags can be incorporated using different strategies: affinity and solubility tags are set individually or together, and sites for protease cleavage are designed between…

Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic,…

CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experimenter with temporal control over…