Author: ARANAX333
How to identify biotinylated proteins? Short fast 1 minute answer!
Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…
How do solubility fusion tags produce soluble proteins? Short fast 1 minute answer!
The ability of solubility fusion tags to produce soluble protein is only the first step in the pathway towards protein production. As many of these…
Does the H tag work as a solubility enhancer? Short fast 1 minute answer!
Regarding the H tag, it did not function as a solubility enhancer tag, but it improved the production levels of target proteins in E. colisimilarly…
Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!
Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.
What is Intein Mediated Purification ? Short fast 1 minute answer!
One of the purification methods used in the biotechnology laboratories for better detection and separation is Intein mediated purification techniques. The intein-mediated purification system has…
What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4
What is a maltose-binding protein? Short fast 1 minute answer!
Maltose binding protein is one of the preparation and separation techniques used in biotechnology laboratories. Maltose-binding protein ( MBP) is a part of the maltose…
How can I incorporate solubility enhancers into my fusion tag design? Short fast 1 minute answer!
Fusion tags can be incorporated using different strategies: affinity and solubility tags are set individually or together, and sites for protease cleavage are designed between…
What are cold shock proteins? Short fast 1 minute answer!
Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic,…
How do you control ectopic gene expression in Drosophila? Short fast 1 minute answer!
CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experimenter with temporal control over…