Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Protein Expression Systems Protein Purification Purification of Recombinant Proteins

How do you purify GST-tagged fusion proteins? Short fast 1 minute answer!

GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Glutathione-based affinity…

Continue Reading... How do you purify GST-tagged fusion proteins? Short fast 1 minute answer!
Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Protein Expression Systems Purification of Recombinant Proteins

Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!

GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GST was…

Continue Reading... Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!
Posted in Articles about Biotechnology Biotechnology Microbiology

Terms and keywords we should be familiar with in Microbiology science

As a microbiologist, we should know series of words and terms that can help us understand details in researches and scientific articles. Abiogenesis Acellular microbe…

Continue Reading... Terms and keywords we should be familiar with in Microbiology science
Posted in Biotinylated Fusion Protein - AviTag Experimental Porotocols in Biotechnology Protein Purification Purification of Recombinant Proteins

How to do biotinylation of fusion protein? Short fast 1 minute answer!

The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification. Including biotin in your culture and inducing protein expression…

Continue Reading... How to do biotinylation of fusion protein? Short fast 1 minute answer!
Posted in Articles about Biotechnology Bioinformatics Biotechnology

Suggestions to study about biotechnology

As a biotechnology students one of big question usually asked is about, where and how we should start reading new subject in our free time…

Continue Reading... Suggestions to study about biotechnology
Posted in Articles about Biotechnology Biotechnology

PRACTICAL APPLICATIONS OF BIOTECHNOLOGY

As a biotechnologist facing lots of facts and theories are interesting but reality of science shows itself when we decide to create a good service…

Continue Reading... PRACTICAL APPLICATIONS OF BIOTECHNOLOGY
Posted in Biotechnology Biotechnology Techniques NMR NMR Websites NMR Websites and Links

BRUKER: A Website to learn about NMR

USA BRUKER, is a website about NMR: They have lots of service and articles about NMR and NMR technologies. Link: https://www.bruker.com/en/products-and-solutions/mr.html They are one of…

Continue Reading... BRUKER: A Website to learn about NMR
Posted in Biotechnology NMR NMR Instruments

BRUKER AvanceCore NMR instrument

The AvanceCore combines Bruker‘s extensive NMR expertise and streamlined production processes in an extremely cost-effective product. It is geared towards customers for whom resource efficiency is paramount. Link:…

Continue Reading... BRUKER AvanceCore NMR instrument
Posted in Microbiology

Careers for microbiologists

Agricultural microbiology is an excellent career field for individuals with interests in both agriculture and microbiology. Included in the field of agricultural microbiology are studies…

Continue Reading... Careers for microbiologists
Posted in Biotechnology Biotechnology Laboratory Equipment

Documentation and the Lab Notebook in Biotechnology

Documentation in a lab notebook is an essential skill for any biotechnician. The Food and Drug Administration’s (FDA) handbook states, “if it isn’t written down,…

Continue Reading... Documentation and the Lab Notebook in Biotechnology