Posted in Bacterial Expression Systems DNA Experimental Porotocols in Biotechnology GST-Fusion Proteins Protein Expression Systems

Tips for Inserting DNA for GST Tagging

Insert foreign GST tag protein sequence in the protein needs a checklist from Primary steps of DNA Engineering to make sure about the latest result…

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Posted in Bacterial Expression Systems Biotechnology Experimental Porotocols in Biotechnology GST-Fusion Proteins pGEX vectors Protein Expression Systems Vector

The host for pGEX vector and GST tagging

GST Tag is one of top techniques in biotechnology laboratory that use as an economic easy strategy for protein separation, purification and detection. The host…

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins Protein Expression Systems

Short review to GST Tag protein

GST Tag protein is one of important amino acid sequences that used for protein engineering for protein purification and protein detection purposes. The GST Tag…

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Posted in Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!

Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology Protein Expression Systems Solubility enhancement tags (SETs)

What is the application of the Solubility enhancement tags (SETs)? Short fast 1 minute answer!

The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and sample stability issues and has enabled structural studies of important biological…

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Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the difference between bioID and biotinylation? Short fast 1 minute answer!

Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase…

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology Protein Expression Systems Solubility enhancement tags (SETs)

How do solubility fusion tags produce soluble proteins? Short fast 1 minute answer!

The ability of solubility fusion tags to produce soluble protein is only the first step in the pathway towards protein production. As many of these…

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Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

How to identify biotinylated proteins? Short fast 1 minute answer!

Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology Protein Protein Expression Systems short article Solubility enhancement tags (SETs)

Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!

Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.

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Posted in Bacterial Expression Systems Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4

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