Category: Experimental Porotocols in Biotechnology
Series of experimental models and working standardized protocols used in Biotechnology and Bioinformatics researches
Tips for Inserting DNA for GST Tagging
Insert foreign GST tag protein sequence in the protein needs a checklist from Primary steps of DNA Engineering to make sure about the latest result…
The host for pGEX vector and GST tagging
GST Tag is one of top techniques in biotechnology laboratory that use as an economic easy strategy for protein separation, purification and detection. The host…
Short review to GST Tag protein
GST Tag protein is one of important amino acid sequences that used for protein engineering for protein purification and protein detection purposes. The GST Tag…
What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!
Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.
What is the difference between bioID and biotinylation? Short fast 1 minute answer!
Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase…
How to identify biotinylated proteins? Short fast 1 minute answer!
Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…
How do solubility fusion tags produce soluble proteins? Short fast 1 minute answer!
The ability of solubility fusion tags to produce soluble protein is only the first step in the pathway towards protein production. As many of these…
Does the H tag work as a solubility enhancer? Short fast 1 minute answer!
Regarding the H tag, it did not function as a solubility enhancer tag, but it improved the production levels of target proteins in E. colisimilarly…
Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!
Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.
What is Intein Mediated Purification ? Short fast 1 minute answer!
One of the purification methods used in the biotechnology laboratories for better detection and separation is Intein mediated purification techniques. The intein-mediated purification system has…