Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the difference between bioID and biotinylation? Short fast 1 minute answer!

Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase…

Continue Reading... What is the difference between bioID and biotinylation? Short fast 1 minute answer!
Posted in Bacterial Expression Systems Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4

Continue Reading... What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!
Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

How to identify biotinylated proteins? Short fast 1 minute answer!

Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…

Continue Reading... How to identify biotinylated proteins? Short fast 1 minute answer!