Posted in Bacterial Expression Systems DNA Experimental Porotocols in Biotechnology GST-Fusion Proteins Protein Expression Systems

Tips for Inserting DNA for GST Tagging

Insert foreign GST tag protein sequence in the protein needs a checklist from Primary steps of DNA Engineering to make sure about the latest result…

Continue Reading... Tips for Inserting DNA for GST Tagging
Posted in Bacterial Expression Systems Biotechnology Experimental Porotocols in Biotechnology GST-Fusion Proteins pGEX vectors Protein Expression Systems Vector

The host for pGEX vector and GST tagging

GST Tag is one of top techniques in biotechnology laboratory that use as an economic easy strategy for protein separation, purification and detection. The host…

Continue Reading... The host for pGEX vector and GST tagging
Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins Protein Expression Systems

Short review to GST Tag protein

GST Tag protein is one of important amino acid sequences that used for protein engineering for protein purification and protein detection purposes. The GST Tag…

Continue Reading... Short review to GST Tag protein
Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Protein Expression Systems Protein Purification Purification of Recombinant Proteins

What is a GST tag? Short fast 1 minute answer!

GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. The GST…

Continue Reading... What is a GST tag? Short fast 1 minute answer!
Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Protein Expression Systems Protein Purification Purification of Recombinant Proteins

How do you purify GST-tagged fusion proteins? Short fast 1 minute answer!

GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Glutathione-based affinity…

Continue Reading... How do you purify GST-tagged fusion proteins? Short fast 1 minute answer!
Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins Protein Protein Expression Systems short article

What is the function of GST fusion protein? Short fast 1 minute answer!

GST fusion protein, is one of highly used techniques in biotechnology laboratories for protein tagging and purification methods. GST is a 211 amino acid protein…

Continue Reading... What is the function of GST fusion protein? Short fast 1 minute answer!
Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Protein Expression Systems Purification of Recombinant Proteins

Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!

GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GST was…

Continue Reading... Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!
Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Expression Systems Protein Purification Purification of Recombinant Proteins

GST fusion protein puriication: top question, Top Answer, Biotechnology course

GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result…

Continue Reading... GST fusion protein puriication: top question, Top Answer, Biotechnology course