Category: GST-Fusion Proteins
A gst fusion protein is a protein that is tagged with GST protein. The fusion protein is made by adding the sequence encoding GST to the plasmid expressing your protein of interest. GST has an affinity for GSH making them a good pair for use in chromatography and immunoprecipitation.
What is a GST tag? Short fast 1 minute answer!
GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. The GST…
Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!
GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GST was…
How do you purify GST-tagged fusion proteins? Short fast 1 minute answer!
GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Glutathione-based affinity…
What is the function of GST fusion protein? Short fast 1 minute answer!
GST fusion protein, is one of highly used techniques in biotechnology laboratories for protein tagging and purification methods. GST is a 211 amino acid protein…
GST fusion protein puriication: top question, Top Answer, Biotechnology course
GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result…
Tips for Inserting DNA for GST Tagging
Insert foreign GST tag protein sequence in the protein needs a checklist from Primary steps of DNA Engineering to make sure about the latest result…
The host for pGEX vector and GST tagging
GST Tag is one of top techniques in biotechnology laboratory that use as an economic easy strategy for protein separation, purification and detection. The host…
Short review to GST Tag protein
GST Tag protein is one of important amino acid sequences that used for protein engineering for protein purification and protein detection purposes. The GST Tag…