The ability of solubility fusion tags to produce soluble protein is only the first step in the pathway towards protein production. As many of these tags are large proteins, it is often necessary to remove the tags after they have been used to make soluble fusion partners.
Category Archives: Solubility enhancement tags (SETs)
Does the H tag work as a solubility enhancer? Short fast 1 minute answer!
Regarding the H tag, it did not function as a solubility enhancer tag, but it improved the production levels of target proteins in E. colisimilarly to the Fh8 tag (Costa et al., 2013a).
Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!
Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.
How can I incorporate solubility enhancers into my fusion tag design? Short fast 1 minute answer!
Fusion tags can be incorporated using different strategies: affinity and solubility tags are set individually or together, and sites for protease cleavage are designed between the fusion tags and target proteins. Solubility enhancer partners
What is the application of the Solubility enhancement tags (SETs)? Short fast 1 minute answer!
The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and sample stability issues and has enabled structural studies of important biological systems previously deemed unapproachable by solution NMR techniques.