How to do biotinylation of fusion protein? Short fast 1 minute answer!

The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification. Including biotin in your culture and inducing protein expression with IPTG results in in vivo biotinylation. The co-expressed ligase will biotinylate the lysine residue in the AviTag.

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