Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Protein Expression Systems Protein Purification Purification of Recombinant Proteins

What is a GST tag? Short fast 1 minute answer!

GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. The GST…

Continue Reading... What is a GST tag? Short fast 1 minute answer!
Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Protein Expression Systems Protein Purification Purification of Recombinant Proteins

How do you purify GST-tagged fusion proteins? Short fast 1 minute answer!

GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Glutathione-based affinity…

Continue Reading... How do you purify GST-tagged fusion proteins? Short fast 1 minute answer!
Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Protein Expression Systems Purification of Recombinant Proteins

Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!

GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GST was…

Continue Reading... Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!
Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Expression Systems Protein Purification Purification of Recombinant Proteins

GST fusion protein puriication: top question, Top Answer, Biotechnology course

GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result…

Continue Reading... GST fusion protein puriication: top question, Top Answer, Biotechnology course