Category: GST-Fusion Proteins
A GST-fusion protein is a protein that is tagged with GST protein. The fusion protein is made by adding the sequence encoding GST to the plasmid expressing your protein of interest. GST has an affinity for GSH making them a good pair for use in chromatography and immunoprecipitation.
What is a GST tag? Short fast 1 minute answer!
GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. The GST…
Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!
GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GST was…
How do you purify GST-tagged fusion proteins? Short fast 1 minute answer!
GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Glutathione-based affinity…
GST fusion protein puriication: top question, Top Answer, Biotechnology course
GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result…