GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. The GST tag. GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result of expression from this …
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GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Glutathione-based affinity purification of GST-tagged fusion proteins is easily done at either small, medium or large scales to produce microgram, milligram or gram quantities. At 26 kDa, GST is considerably larger than …
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GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GST was originally selected as a fusion moiety because of several desirable properties. First and foremost, when expressed in bacteria alone, or as a fusion, GST is not sequestered in inclusion bodies …
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GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result of expression from this vector is a GST-tagged fusion protein in which the functional GST protein (26 kDa) is fused to the N-terminus of the recombinant protein. How does a …
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