Posted in Articles about Biotechnology Biotechnology Protein

A short review to Protein structures: Domains, motifs, and folds

Proteins biophysical an biochemistry features make identity of each protein unique and these units change shape, function and structure protein for special purpose. Proteins are…

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Posted in Articles about Biotechnology Biotechnology Protein

Protein structures: from Amino acid residues to Quaternary Structure

Amino acid residues in proteins: Amino acids are basic structure of proteins in every proteins we know in living organisms and make features and change…

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Posted in Bioinformatics Bioinformatics Websites and Plarforms immunoinformatics Immunoinformatics Website and platforms Protein

A short review to KNOTTIN proteins database

Knottins proteins are a new division of small disulfide-rich proteins characterized by a very special “disulfide through disulfide knot” in a classification of proteins.This knot…

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Posted in Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!

Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology Protein Protein Expression Systems short article Solubility enhancement tags (SETs)

Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!

Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.

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Posted in Protein short article

What are cold shock proteins? Short fast 1 minute answer!

Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic,…

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Posted in Experimental Porotocols in Biotechnology Protein Protein Expression Systems

How do you control ectopic gene expression in Drosophila? Short fast 1 minute answer!

CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experimenter with temporal control over…

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Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the difference between bioID and biotinylation? Short fast 1 minute answer!

Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase…

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Posted in Bacterial Expression Systems Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4

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Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

How to identify biotinylated proteins? Short fast 1 minute answer!

Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…

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