Posted in Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!

Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.

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Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the difference between bioID and biotinylation? Short fast 1 minute answer!

Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase…

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Posted in Protein short article

What are cold shock proteins? Short fast 1 minute answer!

Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic,…

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Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

How to identify biotinylated proteins? Short fast 1 minute answer!

Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology Protein Protein Expression Systems short article Solubility enhancement tags (SETs)

Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!

Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Protein Expression Systems Protein Purification Purification of Recombinant Proteins

What is a GST tag? Short fast 1 minute answer!

GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. The GST…

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Posted in Bacterial Expression Systems Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4

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Posted in Experimental Porotocols in Biotechnology Protein Protein Expression Systems

How do you control ectopic gene expression in Drosophila? Short fast 1 minute answer!

CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experimenter with temporal control over…

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Posted in Bacterial Expression Systems Poly His-Tagged Fusion Proteins poly His-Tagged Fusion Proteins Protein Protein Expression Systems Protein Purification Purification of Recombinant Proteins

What is a Poly His tag Protein? Short fast 1 minute answer!

Poly-His tag protein is one of the sequences that made a big revolution in protein purification and preparation in the biotechnology laboratories. Poly-His tag is…

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins GST-Fusion Proteins Protein Protein Expression Systems Purification of Recombinant Proteins

Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!

GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GST was…

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