Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currRead More…
CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experiRead More…
Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, meRead More…
Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner pRead More…
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred RaidRead More…
Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylatRead More…
Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuRead More…
Maltose binding protein is one of the revolutions in molecular biology for preparation and separation of the protein from samples. It has been repRead More…
Poly-His tag protein is one of the sequences that made a big revolution in protein purification and preparation in the biotechnology laboratories. Read More…
GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Read More…
GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Read More…
GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GRead More…