Category: Protein
Information and articles about protein, protein structure, protein function, protein analysis, protein engineering, protein database, protein software, protein manipulation, novo protein, protein technology, protein search engine
A short review to Protein structures: Domains, motifs, and folds
Proteins biophysical an biochemistry features make identity of each protein unique and these units change shape, function and structure protein for special purpose. Proteins are…
Protein structures: from Amino acid residues to Quaternary Structure
Amino acid residues in proteins: Amino acids are basic structure of proteins in every proteins we know in living organisms and make features and change…
A short review to KNOTTIN proteins database
Knottins proteins are a new division of small disulfide-rich proteins characterized by a very special “disulfide through disulfide knot” in a classification of proteins.This knot…
How to identify biotinylated proteins? Short fast 1 minute answer!
Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…
What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!
Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.
What are cold shock proteins? Short fast 1 minute answer!
Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic,…
What is the difference between bioID and biotinylation? Short fast 1 minute answer!
Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase…
Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!
Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.
What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4
How do you control ectopic gene expression in Drosophila? Short fast 1 minute answer!
CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experimenter with temporal control over…