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Category: Protein

Information and articles about protein, protein structure, protein function, protein analysis, protein engineering, protein database, protein software, protein manipulation, novo protein, protein technology, protein search engine

What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!

Leave a Comment on What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!

Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currRead More…

How do you control ectopic gene expression in Drosophila? Short fast 1 minute answer!

Leave a Comment on How do you control ectopic gene expression in Drosophila? Short fast 1 minute answer!

CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experiRead More…

What are cold shock proteins? Short fast 1 minute answer!

Leave a Comment on What are cold shock proteins? Short fast 1 minute answer!

Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, meRead More…

Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!

Leave a Comment on Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!

Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner pRead More…

What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!

Leave a Comment on What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred RaidRead More…

How to identify biotinylated proteins? Short fast 1 minute answer!

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Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylatRead More…

What is the difference between bioID and biotinylation? Short fast 1 minute answer!

Leave a Comment on What is the difference between bioID and biotinylation? Short fast 1 minute answer!

Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuRead More…

Is it possible to crystallize target proteins by fusion of maltose-binding proteins? Short fast 1 minute answer!

Leave a Comment on Is it possible to crystallize target proteins by fusion of maltose-binding proteins? Short fast 1 minute answer!

Maltose binding protein is one of the revolutions in molecular biology for preparation and separation of the protein from samples. It has been repRead More…

What is a Poly His tag Protein? Short fast 1 minute answer!

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Poly-His tag protein is one of the sequences that made a big revolution in protein purification and preparation in the biotechnology laboratories. Read More…

What is a GST tag? Short fast 1 minute answer!

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GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Read More…

How do you purify GST-tagged fusion proteins? Short fast 1 minute answer!

Leave a Comment on How do you purify GST-tagged fusion proteins? Short fast 1 minute answer!

GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Read More…

Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!

Leave a Comment on Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!

GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GRead More…

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