Proteins biophysical an biochemistry features make identity of each protein unique and these units change shape, function and structure protein for special purpose. Proteins are…

Amino acid residues in proteins: Amino acids are basic structure of proteins in every proteins we know in living organisms and make features and change…

Knottins proteins are a new division of small disulfide-rich proteins characterized by a very special “disulfide through disulfide knot” in a classification of proteins.This knot…

Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.

Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.

Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic,…

CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experimenter with temporal control over…

Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase…

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4

Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…