Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.
Category Archives: Protein
What is the difference between bioID and biotinylation? Short fast 1 minute answer!
Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase and stably expressed in cells; the fusion protein biotinylated proximal proteins only when excess biotin is added to the medium. High-affinity avidin/streptavidin-beads are used to capture biotinylated candidates in a …
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What are cold shock proteins? Short fast 1 minute answer!
Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic, thermophilic and even hyperthermophilic bacteria.
How to identify biotinylated proteins? Short fast 1 minute answer!
Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate vicinal proteins upon supplementation of the culture medium with biotin. Biotinylated proteins can then be selectively isolated and identified by conventional methods, most notably mass spectrometry.
Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!
Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.
What is a GST tag? Short fast 1 minute answer!
GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. The GST tag. GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result of expression from this …
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What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4
How do you control ectopic gene expression in Drosophila? Short fast 1 minute answer!
CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experimenter with temporal control over transgene induction, or the GAL4-UAS system, which provides the experimenter with spatial control over transgene expression.
What is a Poly His tag Protein? Short fast 1 minute answer!
Poly-His tag protein is one of the sequences that made a big revolution in protein purification and preparation in the biotechnology laboratories. Poly-His tag is the most commonly used method for protein purification and preparation and can be used in almost all expression systems: bacteria, yeast, mammalian, and insect systems. Elution agent imidazole may affect …
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Why was GST originally selected as a fusion moiety? Short fast 1 minute answer!
GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GST was originally selected as a fusion moiety because of several desirable properties. First and foremost, when expressed in bacteria alone, or as a fusion, GST is not sequestered in inclusion bodies …
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