A short review to Protein structures: Domains, motifs, and folds

Proteins biophysical an biochemistry features make identity of each protein unique and these units change shape, function and structure protein for special purpose. Proteins are usually known as consisting of multiple structural units. These units include domains, motifs, and folds. With this vision that there are about 100,000 different proteins expressed in eukaryotic living organisms, …

Protein structures: from Amino acid residues to Quaternary Structure

Amino acid residues in proteins: Amino acids are basic structure of proteins in every proteins we know in living organisms and make features and change in protein structures. Each Amino acid consists of a backbone that is present in all the amino acid types and a side chain that is unique to each type of …

A short review to KNOTTIN proteins database

Knottins proteins are a new division of small disulfide-rich proteins characterized by a very special “disulfide through disulfide knot” in a classification of proteins.This knot is achieved when one disulfide bridge crosses the macrocycle formed by the two other disulfides and the interconnecting backbone.The knot structure implies that knottins contain at least 3 disulfide bridges …

How to identify biotinylated proteins? Short fast 1 minute answer!

Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate vicinal proteins upon supplementation of the culture medium with biotin. Biotinylated proteins can then be selectively isolated and identified by conventional methods, most notably mass spectrometry.

What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!

Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.

What is the difference between bioID and biotinylation? Short fast 1 minute answer!

Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase and stably expressed in cells; the fusion protein biotinylated proximal proteins only when excess biotin is added to the medium. High-affinity avidin/streptavidin-beads are used to capture biotinylated candidates in a …

Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!

Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.

What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4

How do you control ectopic gene expression in Drosophila? Short fast 1 minute answer!

CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experimenter with temporal control over transgene induction, or the GAL4-UAS system, which provides the experimenter with spatial control over transgene expression.

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