GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. The GST tag. GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result of expression from this …
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GST fusion protein, is one of highly used techniques in biotechnology laboratories for protein tagging and purification methods. GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result of expression from this vector is a GST-tagged fusion protein in which …
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Maltose binding protein is one of the revolutions in molecular biology for preparation and separation of the protein from samples. It has been reported repeatedly by many labs to make crystallization of target proteins possible only by fusion of maltose-binding protein (MBP) to the N-terminus of their targets.
Poly-His tag protein is one of the sequences that made a big revolution in protein purification and preparation in the biotechnology laboratories. Poly-His tag is the most commonly used method for protein purification and preparation and can be used in almost all expression systems: bacteria, yeast, mammalian, and insect systems. Elution agent imidazole may affect …
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GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. Glutathione-based affinity purification of GST-tagged fusion proteins is easily done at either small, medium or large scales to produce microgram, milligram or gram quantities. At 26 kDa, GST is considerably larger than …
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GST fusion protein, is a tagging method that will fuse amino acid sequence to the protein structure for the purification and separation methods. GST was originally selected as a fusion moiety because of several desirable properties. First and foremost, when expressed in bacteria alone, or as a fusion, GST is not sequestered in inclusion bodies …
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Part I Introduction This group focuses on fundamental and applied aspects of industrial microbiology, especially in amino acid production. Research works include investigation on the mechanism of mutation of amino acid-producing mutants, strain improvement by genetic engineering and metabolic engineering, and investigation on the metabolic pathway of cyclic imide in microorganism. This group has 4 …
Usual techniques and subjects that are repeatedly use and study in Genomics and Proteomics researches are listed here, if you want to start your business or design your study roadmap, this short note will help you understand your way. Organization and Structure of Genome Gene mapping Transcript mapping Gene expression and regulation RNA interference Protein …
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