Biotechnology experts usually use daily and hourly these techniques for analyzing and understanding molecule content of life. These technqieus are highly usage techniques in biotechnology listed here:

Recombinant DNA technology
Protein and nucleic acid gel electrophoresis
Blotting techniques (Western, Nothren and Southren Blotting)
Restriction endonucleases
Restriction mapping
Vectors and their types
Polymerase Chain reaction (PCR) and its type
Cloning strategies
Site-directed mutagenesis
Sequencing techniques
Immunohistochemisty
Immunocytochemisty
Microscopy (light, confocal and electron)
Chromatographic techniques (TLC, Paper Chromatography, Gel filteration, ion exchange; affinity chromatography, HPLC, GC)
ELISA