Posted in Bacterial Expression Systems DNA Experimental Porotocols in Biotechnology GST-Fusion Proteins Protein Expression Systems

Tips for Inserting DNA for GST Tagging

Insert foreign GST tag protein sequence in the protein needs a checklist from Primary steps of DNA Engineering to make sure about the latest result…

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Posted in Bacterial Expression Systems Biotechnology Experimental Porotocols in Biotechnology GST-Fusion Proteins pGEX vectors Protein Expression Systems Vector

The host for pGEX vector and GST tagging

GST Tag is one of top techniques in biotechnology laboratory that use as an economic easy strategy for protein separation, purification and detection. The host…

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology GST-Fusion Proteins Protein Expression Systems

Short review to GST Tag protein

GST Tag protein is one of important amino acid sequences that used for protein engineering for protein purification and protein detection purposes. The GST Tag…

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Posted in Articles about Biotechnology Biotechnology Protein

A short review to Protein structures: Domains, motifs, and folds

Proteins biophysical an biochemistry features make identity of each protein unique and these units change shape, function and structure protein for special purpose. Proteins are…

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Posted in Articles about Biotechnology Biotechnology Protein

Protein structures: from Amino acid residues to Quaternary Structure

Amino acid residues in proteins: Amino acids are basic structure of proteins in every proteins we know in living organisms and make features and change…

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Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

How to identify biotinylated proteins? Short fast 1 minute answer!

Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…

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Posted in Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!

Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology Protein Expression Systems Solubility enhancement tags (SETs)

How do solubility fusion tags produce soluble proteins? Short fast 1 minute answer!

The ability of solubility fusion tags to produce soluble protein is only the first step in the pathway towards protein production. As many of these…

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Posted in Protein short article

What are cold shock proteins? Short fast 1 minute answer!

Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic,…

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Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the difference between bioID and biotinylation? Short fast 1 minute answer!

Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase…

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