Tag: Protein Engineering
Protein engineering is the conception and production of unnatural polypeptides, often through modification of amino acid sequences that are found in nature. Synthetic protein structures and functions can now be designed entirely on a computer or produced through directed evolution in the laboratory.
Tips for Inserting DNA for GST Tagging
Insert foreign GST tag protein sequence in the protein needs a checklist from Primary steps of DNA Engineering to make sure about the latest result…
The host for pGEX vector and GST tagging
GST Tag is one of top techniques in biotechnology laboratory that use as an economic easy strategy for protein separation, purification and detection. The host…
Short review to GST Tag protein
GST Tag protein is one of important amino acid sequences that used for protein engineering for protein purification and protein detection purposes. The GST Tag…
A short review to Protein structures: Domains, motifs, and folds
Proteins biophysical an biochemistry features make identity of each protein unique and these units change shape, function and structure protein for special purpose. Proteins are…
Protein structures: from Amino acid residues to Quaternary Structure
Amino acid residues in proteins: Amino acids are basic structure of proteins in every proteins we know in living organisms and make features and change…
How to identify biotinylated proteins? Short fast 1 minute answer!
Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…
What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!
Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.
How do solubility fusion tags produce soluble proteins? Short fast 1 minute answer!
The ability of solubility fusion tags to produce soluble protein is only the first step in the pathway towards protein production. As many of these…
What are cold shock proteins? Short fast 1 minute answer!
Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic,…
What is the difference between bioID and biotinylation? Short fast 1 minute answer!
Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase…