Tag: Protein Engineering
Tips for Inserting DNA for GST Tagging
Insert foreign GST tag protein sequence in the protein needs a checklist from Primary steps of DNA Engineering to make sure about the latest result…
The host for pGEX vector and GST tagging
GST Tag is one of top techniques in biotechnology laboratory that use as an economic easy strategy for protein separation, purification and detection. The host…
Short review to GST Tag protein
GST Tag protein is one of important amino acid sequences that used for protein engineering for protein purification and protein detection purposes. The GST Tag…
A short review to Protein structures: Domains, motifs, and folds
Proteins biophysical an biochemistry features make identity of each protein unique and these units change shape, function and structure protein for special purpose. Proteins are…
Protein structures: from Amino acid residues to Quaternary Structure
Amino acid residues in proteins: Amino acids are basic structure of proteins in every proteins we know in living organisms and make features and change…
What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!
Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.
Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!
Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.
How do solubility fusion tags produce soluble proteins? Short fast 1 minute answer!
The ability of solubility fusion tags to produce soluble protein is only the first step in the pathway towards protein production. As many of these…
What are cold shock proteins? Short fast 1 minute answer!
Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic,…
What is the application of the Solubility enhancement tags (SETs)? Short fast 1 minute answer!
The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and sample stability issues and has enabled structural studies of important biological…