Tips for Inserting DNA for GST Tagging

Insert foreign GST tag protein sequence in the protein needs a checklist from Primary steps of DNA Engineering to make sure about the latest result in protein. These are important key for high quality GST tagging process. Insert DNA must possess an open reading frame and should be less than 2 kb long to make …

The host for pGEX vector and GST tagging

GST Tag is one of top techniques in biotechnology laboratory that use as an economic easy strategy for protein separation, purification and detection. The host for this process is important for FDA approved and top resolution result. E. coli as a pioneer organism used in the laboratory for bio engineering is one of the main …

Short review to GST Tag protein

GST Tag protein is one of important amino acid sequences that used for protein engineering for protein purification and protein detection purposes. The GST Tag known as The Glutathione S-transferase (GST) is a Gene Fusion System that use for the expression, purification, and detection of fusion proteins produced in Eschericia coli and FDA approved organism …

A short review to Protein structures: Domains, motifs, and folds

Proteins biophysical an biochemistry features make identity of each protein unique and these units change shape, function and structure protein for special purpose. Proteins are usually known as consisting of multiple structural units. These units include domains, motifs, and folds. With this vision that there are about 100,000 different proteins expressed in eukaryotic living organisms, …

Protein structures: from Amino acid residues to Quaternary Structure

Amino acid residues in proteins: Amino acids are basic structure of proteins in every proteins we know in living organisms and make features and change in protein structures. Each Amino acid consists of a backbone that is present in all the amino acid types and a side chain that is unique to each type of …

How to identify biotinylated proteins? Short fast 1 minute answer!

Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate vicinal proteins upon supplementation of the culture medium with biotin. Biotinylated proteins can then be selectively isolated and identified by conventional methods, most notably mass spectrometry.

What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!

Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.

How do solubility fusion tags produce soluble proteins? Short fast 1 minute answer!

The ability of solubility fusion tags to produce soluble protein is only the first step in the pathway towards protein production. As many of these tags are large proteins, it is often necessary to remove the tags after they have been used to make soluble fusion partners.

What is the difference between bioID and biotinylation? Short fast 1 minute answer!

Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase and stably expressed in cells; the fusion protein biotinylated proximal proteins only when excess biotin is added to the medium. High-affinity avidin/streptavidin-beads are used to capture biotinylated candidates in a …

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