Tag: Protein Engineering
Protein engineering is the conception and production of unnatural polypeptides, often through modification of amino acid sequences that are found in nature. Synthetic protein structures and functions can now be designed entirely on a computer or produced through directed evolution in the laboratory.
How can I incorporate solubility enhancers into my fusion tag design? Short fast 1 minute answer!
Fusion tags can be incorporated using different strategies: affinity and solubility tags are set individually or together, and sites for protease cleavage are designed between…
How to identify biotinylated proteins? Short fast 1 minute answer!
Analogous to DamID, the biotin ligase is fused to a protein of interest, and then introduced into mammalian (or other) cells where it will biotinylate…
Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!
Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.
What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4
How do you control ectopic gene expression in Drosophila? Short fast 1 minute answer!
CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experimenter with temporal control over…
What is the function of maltose/maltodextrin? Short fast 1 minute answer!
Maltose binding protein is one of the revolutions in molecular biology for preparation and separation of the protein from samples. The maltose/maltodextrin is a complex…
What is a GST tag? Short fast 1 minute answer!
GST amino acid sequence used as a method for fuse with our target protein for better purification and separation in the biotechnology laboratories. The GST…
What is the function of a maltose binding protein in E coli? Short fast 1 minute answer!
Maltose binding protein is one of the preparation and separation techniques used in biotechnology laboratories. Maltose Binding Protein is a member of the maltose/maltodextrin system…
What is a Poly His tag Protein? Short fast 1 minute answer!
Poly-His tag protein is one of the sequences that made a big revolution in protein purification and preparation in the biotechnology laboratories. Poly-His tag is…
What is the application of the Solubility enhancement tags (SETs)? Short fast 1 minute answer!
The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and sample stability issues and has enabled structural studies of important biological…