Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and...
Protein
CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of...
Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino...
The ability of solubility fusion tags to produce soluble protein is only the first...
Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have...
Regarding the H tag, it did not function as a solubility enhancer tag, but...
Fusion tags can be incorporated using different strategies: affinity and solubility tags are set...
The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and...
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells...
Analogous to DamID, the biotin ligase is fused to a protein of interest, and...
Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID,...
The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for...