Posted in Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the best binary expression system for drosophilagenetics? Short fast 1 minute answer!

Binary expression systems: GAL4-UAS, LexA-lexAopand QF-QUASsystems The GAL4-UASsystem is the workhorse of Drosophilagenetics, and few papers are currently published that do not use it.

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology Protein Protein Expression Systems short article Solubility enhancement tags (SETs)

Do small peptide tags stimulate solubility in proteins? Short fast 1 minute answer!

Small peptide tags called SET tags, which feature highly acidic amino acid sequences, have also been shown to stimulate solubility in a few partner proteins.

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology Protein Expression Systems Solubility enhancement tags (SETs)

How do solubility fusion tags produce soluble proteins? Short fast 1 minute answer!

The ability of solubility fusion tags to produce soluble protein is only the first step in the pathway towards protein production. As many of these…

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Posted in Protein short article

What are cold shock proteins? Short fast 1 minute answer!

Cold shock proteins are small nucleic acid-binding proteins ranging from 65 to 75 amino acids in length and they have been found in psychrophilic, mesophilic,…

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology Protein Expression Systems Solubility enhancement tags (SETs)

What is the application of the Solubility enhancement tags (SETs)? Short fast 1 minute answer!

The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and sample stability issues and has enabled structural studies of important biological…

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Posted in Experimental Porotocols in Biotechnology Protein Protein Expression Systems

How do you control ectopic gene expression in Drosophila? Short fast 1 minute answer!

CUntil recently, ectopic gene expression in Drosophila was largely accomplished through the use of the heat-shock promoter, which provides the experimenter with temporal control over…

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Posted in Bacterial Expression Systems Biotechnology Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is the difference between bioID and biotinylation? Short fast 1 minute answer!

Biotinylation protein is one technique used in biotechnology for tagging the proteins. In BioID, the protein of interest is fused to a “promiscuous” biotin ligase…

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Posted in Bacterial Expression Systems Biotinylated Fusion Protein Experimental Porotocols in Biotechnology Protein Protein Expression Systems

What is a promiscuous biotin ligase fusion protein? Short fast 1 minute answer!

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells Kyle J. Roux,1,2Dae In Kim,1,2Manfred Raida,3and Brian Burke4

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Posted in Bacterial Expression Systems Experimental Porotocols in Biotechnology Protein Expression Systems Solubility enhancement tags (SETs)

How can I incorporate solubility enhancers into my fusion tag design? Short fast 1 minute answer!

Fusion tags can be incorporated using different strategies: affinity and solubility tags are set individually or together, and sites for protease cleavage are designed between…

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Posted in Bacterial Expression Systems Biotechnology Experimental Porotocols in Biotechnology Protein Expression Systems short article Solubility enhancement tags (SETs)

Does the H tag work as a solubility enhancer? Short fast 1 minute answer!

Regarding the H tag, it did not function as a solubility enhancer tag, but it improved the production levels of target proteins in E. colisimilarly…

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