Students and researchers who are looking for a good biotechnology course, usually ask a question that What are good techniques and skills teach in a profession course in biotechnology.
Usually, Biotechnology Program has been designed to match the needs of the biotechnology job market in our immediate area such as industries and R&D. These courses will invited industrial partners from their own region community to contribute to the competency goals for each course, including this introductory course, to assure that students are adequately prepared for positions in their companies. The following list describes the areas of expertise that you will be introduced to in this course, and may provide you with an organizational plan for the archiving of your records in your notebooks for this course. Here are a lists of courses details such as skills and techniques that make a good biotechnologist at last.
Basic operations in the laboratory (Lab skills)
Purpose:
There are special approaches and precautions that must be taken in any biological laboratory. This includes procedures for safe handling and storage of hazardous chemicals and biologicals. Also, the special methods for setting up and following detailed protocols are emphasized, as well as methods for recording and archiving results properly.
Includes:
Safety in the Laboratory
Math Skills for the Laboratory
Documentation and the Lab Notebook
Molar Solutions and Dilutions
Lab Safety Procedures
Hints for Solving Numerical Problems
Summary of Chemical Hazards, MSDS etc
Hazardous Waste Program etc
Graphing Data
Summary of Good Laboratory Practices
Agarose Gel Electrophoresis with Ethidium Bromide
Instruments and Equipment in laboratory:
Purpose:
An important part of working in any laboratory is the proper use and calibration of instruments and equipment. You will become familiar with general information about the use of lab equipment, as well as more detailed information about the step-by-step procedures for the specific instruments that you use.
Includes:
Basic Tools in the Biotechnology Laboratory
Specialized Biotech Program Equipment locator
Using a Micropipetter
micropipetters
Calibrating Lab Instruments
balances and pH meters
Restriction Enzyme Mapping of DNA
agarose gel electrophoresis
GFP Chromatography
denaturing polyacrylamide gel electrophoresis
DNA Fingerprinting: Alu PCR
thermal cycler, agarose gel electrophoresis
Working with DNA and proteins
Purpose:
It is important to be familiar with the basic techniques for purifying and analyzing biomolecules. You will learn to isolate, digest, and analyze DNA, as well as transform E. coli with a recombinant plasmid. You will also learn some basic methods to purify and analyze proteins.
Includes:
Transformation of E. coli
in vivo amplification of plasmid DNA
Plasmid Isolation
isolation of DNA
Restriction Enzyme Mapping of DNA
analysis of a restriction digest
DNA Fingerprinting: Alu PCR
isolation of genomic DNA, in vitro amplification
of DNA by polymerase chain reaction
GFP Chromatography
hydrophobic interaction chromatography,
polyacrylamide gel electrophoresis
Immunochemistry
Purpose:
You will be introduced to basic techniques used to detect biomolecules using antibodies.
Includes:
ELISA for HIV
Enzyme-linked immunosorbent assay
Environmental microbiology
Regulatory Affairs
Purpose:
You will work on writing skills and how to follow Standard Operating Procedures (SOPs) in the laboratory. The regulations governing biological laboratories dictate the safety procedures and protocols for disposal of hazardous chemicals and biologicals.
Includes:
Documentation and the Lab Notebook
Lab Safety Procedures
Summary of Chemical Hazards, MSDS
Purpose:
You will use microbes to remove environmental pollutants.
Includes:
Bioremediation:
Environmental Clean-Up
Bioinformatics
Purpose:
Using computers to document and compile information is becoming the norm in biological laboratories. Computers are also used to access databases with genomic or statistical information. Your instructor will decide on the appropriate tutorials.