Poly-His tag protein is one of the sequences that made a big revolution in protein purification and preparation in the biotechnology laboratories.

Poly-His tag is the most commonly used method for protein purification and preparation and can be used in almost all expression systems: bacteria, yeast, mammalian, and insect systems. Elution agent imidazole may affect downstream studies like NMR, competition studies, and X-ray crystallography, and may induce protein aggregation.

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